MicroRNA Detection and Target Identification

图书简介

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Detection of miRNAs.- MicroRNA Identification, Target Prediction, and Validation for Crop Improvement.- MicroRNA Detection with CRISPR/Cas.- Detection of MicroRNAs by Northern Blot.- MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore.- Detection of MicroRNAs Expression Dynamics Using LNA/DNA Nanobiosensor.- Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification.- Small RNA Profiling by Next Generation Sequencing Using High-Definition Adapters.- Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification.- Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine.- Determining miRNA Expression Patterns in Xenopus.- Overview of Computational and Experimental Methods to Identify Tissue-Specific MicroRNA Targets.- sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non-Model Species.- MicroRNA-Target Identification: A Combinatorial In Silico Approach.- An Efficient CRISPR-Cas9 Method to Knockout miRNA Expression in Xenopus tropicalis.- Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

MicroRNA Detection and Target Identification（Methods in Molecular Biology） 微小核糖核酸检测与目标识别：方法与协议 第2版 遗传学

MicroRNA Detection and Target Identification（Methods in Molecular Biology）

微小核糖核酸检测与目标识别：方法与协议第2版
遗传学