图书简介

Make it shine: Labelling the ER for light and fluorescence microscopy.- Electron microscopy techniques for 3D plant ER imaging.- Studying plant ER-PM contact site localised proteins using microscopy.- Preparation and imaging of specialised ER using super-resolution and TEM techniques.- Quantitation of ER morphology and dynamics.- Long-term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination.- Dancing with the stars: Using image analysis to study the choreography of the endoplasmic reticulum and its partners and of movement within its tubules.- Preparation of highly enriched ER membranes using free-flow electrophoresis.-Preparation of ER microsomes from Arabidopsis thaliana.- ER membrane lipid composition and metabolism: lipidomic analysis.- 2in1 vectors improve in planta BiFC and FRET analysis.- Immunoprecipitation and FRET-FLIM to determine metabolons on the plant ER.- Using optical tweezers combined with total internal reflection microscopy to study interactions between the ER and Golgi in plant cells.- Protein biosynthesis and maturation in the ER.- ER membrane Protein Interactions using the Split-Ubiquitin System (SUS).- Analysis of protein glycosylation in the ER.- Unfolded Protein Response in Arabidopsis.- Imaging the ER and endomembrane system in cereal endosperm.- Multi-omics resources for understanding gene regulation in response to ER stress in plants.- Variable angle epifluorescence microscopy for single particle tracking in the plant ER.- Use of super resolution live cell imaging to distinguish endoplasmic reticulum - nuclear envelope subcellular localisation.- Using ER-targeted photoconvertible fluorescent proteins in living plant cells.- In vivo analysis of ER-associated protein degradation and ubiquitination in Arabidopsis thaliana.- Overexpression of endoplasmic reticulum proteins from Arabidopsis thaliana in baculovirus.- Observing ER dynamics over long timescales using light sheet fluorescence microscopy.- Gene stacking and stoichiometric expression of ER-targeted constructs using “2A” self-cleaving peptides.- Automated Segmentation and 3D Reconstruction of Different Membranes from Confocal Z-stacks.- Visualizing orientation and topology of ER membrane proteins in planta.- Organ specific microsomes from dark grown hypocotyls of Arabidopsis thaliana.- Studying secretory protein synthesis in Nicotiana benthamiana protoplasts.- Analyzing photoactivation with diffusion models to study transport in the Endoplasmic Reticulum network.

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