图书简介
With over 820 million people facing hunger in today’s world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc. Key features of the book:Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/CasIntroduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases)Covers the history, origin, discovery, structure, and classification of CRISPR/CasExamines the databases and computational approaches of CRISPR/CasHighlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruitsExplores potential applications of CRISPR/Cas systems for climate smart crops
PART I: INTRODUCTION TO GENOME EDITING TOOLS
1. Conventional Breeding, Genetically Modified Crops, and Genome Editing
Zulqurnain Khan
2. An Overview of Tools for Genome Editing: ZFNs, Mega Nucleases, and Talens
Lekha Bhagtaney and Priya Sundarrajan
3. Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated (CRISPR/Cas) Systems: Discovery, Structure, Classification, and General Mechanism
Suman Ganger, Geetanjali Harale, and Parama Majumdar
4. Computational Tools and Approaches for CRISPR-Cas Technology
Humaira Shah, Auqib Manzoor, and Ashraf Dar
PART II: GENOME EDITING OF CROP PLANTS WITH CRISPR/CAS SYSTEMS: PAST, PRESENT, AND FUTURE
5. Genome Editing Using CRISPR/Cas9 System for Crop Improvement, Nutrient Enrichment, and Abiotic Stress Tolerance in Rice
Aditya Banerjee and Aryadeep Roychoudhury
6. Genome Editing of Wheat with CRISPR/Cas Systems: Past, Present, and Future
Suman Bakshi, Uttam Kumar, Pradeep Bhatti, and Sanjay J. Jambhulkar
7. Genome Editing of Maize with CRISPR/Cas System: Past, Present, and Future
Bushra Hafeez Kiani
8. Genome Editing of Tomato Crop with CRISPR/Cas Systems: Past, Present, and Future
Vincent Ezin and Rachael C. Symonds
9. Genome Editing Techniques and Their Applications in Brassica Crops Improvement
Sanjay J. Jambhulkar, Archana Rai, Shankar K. Bhujbal, and Chinmaya Das
PART III: POTENTIAL APPLICATIONS OF CRISPR/CAS SYSTEMS FOR CLIMATE SMART CROPS
10. CRISPR/Cas-Mediated Genome Editing for Abiotic Stress Tolerance
Farhana Hassan, Amrina Shafi, Umer Majeed Wani, and Firdous A. Khanday
11. CRISPR/Cas-Mediated Genome Editing for Biotic Stress Tole
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