图书简介
This volume covers a broad range of methods, technologies, and protocols on malaria. Chapters detail research on collecting parasites in the field, single molecule-level analyses of adhesive interactions, and focused studies aiming at disrupting the devastating disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Malaria Immunology: Targeting the Surface of Infected Erythrocytes aims to be a useful and practical guide to researches to help further their study in this field. Chapter Analysis of var gene transcription pattern using DBLα-tags [Chapter 14] is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Identification and enumeration of Plasmodium falciparum parasites by light microscopy.- Collection and cryopreservation of Plasmodium falciparum clinical isolates in the field.- Preservation of parasite RNA in the field.- Preservation and extraction of malaria parasite DNA from dried blood spots.- Establishing and maintaining in vitro cultures of asexual blood stages of Plasmodium falciparum.- Genotyping of Plasmodium falciparum isolates to assess clone composition in parasite cultures.- Immunomagnetic selection of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants.- Single-cell sorting of Plasmodium falciparum-infected erythrocytes expressing particular PfEMP1 variants.- Selecting Plasmodium falciparum infected erythrocytes for adhesion to cell lines.- Synchronisation of Plasmodium falciparum and P. knowlesi in vitro cultures using a highly specific protein kinase inhibitor.- Generation of Plasmodium falciparum gametocytes in vitro with specific considerations for field isolates.- Imaging of extracellular vesicles derived from Plasmodium falciparum-infected red blood cells using atomic force microscopy.- Analysis of var gene transcript patterns by quantitative real-time PCR.- Analysis of var gene transcription pattern using DBLα-tags.- RNAseq of infected erythrocyte surface antigen-encoding genes.- Resetting var gene transcription in Plasmodium falciparum.- CRISPR/Cas9 editing of the Plasmodium falciparum genome.- CRISPR-Cas9 editing of the Plasmodium falciparum genome - Special applications.- Extraction and immunoprecipitation of VAR2CSA, the PfEMP1 associated with placental malaria.- Expression of single-domain soluble and disulfide-folded PfEMP1 antigens in the Escherichia coli SHuffle expression system.- Expression of large full-length PfEMP1 proteins in HEK293 cells.- Receptor affinity-based purification of PfEMP1 proteins.- Analysis of antibody reactivity to malaria antigens by microsphere-based multiplex immunoassay.- High-throughput BioPlex assay for the study of functionally active Plasmodium falciparum antigens that are expressed on the surface of infected erythrocytes.- Protein microarrays as a tool to analyze antibody responses to variant surface antigens expressed on the surface of Plasmodium falciparum-infected erythrocytes.- Protocol for differential biopanning of Plasmodium falciparum phage display cDNA library to identify parasite targets of protective antibodies.- Affinity purification of PfEMP1-specific antibodies from human blood.- Production of anti-PfEMP1 polyclonal antisera in rats and mice.- Production of PfEMP1-specific mouse monoclonal antibodie.- Production of PfEMP1-specific human monoclonal antibodies from naturally immune individuals.- Visualization of infected red blood cell surface antigens by fluorescence microscop.- Analysis by flow cytometry of α2‑macroglobulin and non-immune IgM-binding to Plasmodium falciparum-infected erythrocytes.- An improved method for assessing antigen presentation on the surface of Plasmodium falciparum-infected erythrocytes by immuno-electron microscopy.- Assessing antigen presentation on the surface of Plasmodium falciparum-infected erythrocytes by photoactivated localization microscopy (PALM).- Surface plasmon resonance analysis of PfEMP1 interaction with receptors.- Analysis of antibody neutralization of PfEMP1 binding by competition ELISA.- Measuring rosetting inhibition in Plasmodium falciparum parasites using a flow cytometry-based assay.- Bridging and clumping: investigating platelet interactions with Plasmodium falciparum-infected red blood cells and endothelial cells in cerebral malaria.- Assay of static adhesion of Plasmodium falciparum-infected erythrocytes to cel
Trade Policy 买家须知
- 关于产品：
- ● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
- ● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
- ● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：- 由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
- 签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
- ● 缺页/错页/错印/脱线
关于发货时间：- 一般情况下：
- ●【现货】 下单后48小时内由北京（库房）发出快递。
- ●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
- ● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
- ● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：- 由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间：- 010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
- 其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：- ● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐
