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1. Protocols to induce and study ciliogenesis Jose Manuel M. Bravo San Pedro 2. A Flow Cytometry-Based Approach for the study of primary Cilia Francesca Ciccolini 3. Microscopic observation of human airway ciliary movement using wheat germ agglutinin Yo Kishimoto 4. Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins Koji Ikegami 5. Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method Yumiko Saito and Yuki Kobayashi 6. High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathies Tatsuo Miyamoto, Kosuke Hosoba, Tomoka Morita, Ying Zhang, Hiroko Kishi and Takashi Yamamoto 7. Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish Ruxandra Bachman-Gagescu 8. Visualizing Multiciliated Cells in the Zebrafish Rebecca Ann Wingert 9. Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas Stephen M. King 10. Using Paramecium as a Model for Ciliopathies Judith Van Houten 11. Using organoids to study cilia Vincent GUEN 12. Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model Tang K. Tang, Ting-Yu Chen and Chia-Hsiang Chang

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