图书简介
This volume covers comprehensive methods on ways to assess structural and ultrastructural changes in the mitochondria, cytoskeleton, and microglia using state-of-the-art microscopy techniques including super-resolution imaging, electron microscopy, and ultra-high field MRI. The chapters in this book cover topics such as analysis of neurodegeneration in the post-mortem characterization of preclinical animal models, in vivo modeling in cell death in different model systems and brain organoids, single cell clonal analysis using Mosaic Analysis with Double Markers in genetic mouse models, and genome and proteomic methods for analysis of mRNA dynamics and quantitation of targeted peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Neuronal Cell Death: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.
Monitoring Mitochondrial Respiration in Mouse Cerebellar Granule Neurons.- Examining Mitochondrial Morphology in Mouse Brains.- Investigating Microglial Ultrastructural Alterations and Intimate Relationships with Neuronal Stress, Dystrophy, and Degeneration in Mouse Models of Alzheimer’s Disease.- Performing Single Cell Clonal Analysis in the Mouse Brain using Mosaic Analysis with Double Markers (MADM).- Combination of Endothelin-1 (ET-1) and L-NAME to Induce Murine Focal Murine Cortical Stroke with Persistent Sensorimotor Deficit.- Modeling Axonal Degeneration Using Motor Nerve Organoids.- Monitoring Autophagy in Neural Stem and Progenitor Cells.- Direct FACS Isolation of Neural Stem/Progenitor Lineages from the Adult Brain.- Analysis of mRNA Dynamics using RNA Sequencing Data.- Assessment of Dopaminergic Neurodegeneration in Mice.- Super-Resolution Imaging of Cytoskeletal Networks in Fixed Brain Tissue.- Spinal Cord Injury in the Mouse using the Infinite Horizons Spinal Cord Impactor.- Immuno-MALDI-MS for Accurate Quantitation of Targeted Peptides from Volume-Restricted Samples.- Three-Dimensional Atlas of the Human Amygdala Subnuclei Constructed using Immunohistochemical and Ultra-High Field Magnetic Resonance Imaging Data.- Using Single-Molecule Fluorescence Microscopy to Uncover Neuronal Vulnerability to Protein Damage.- Autopsy Human Brain Dissection Protocol for Common Age-Related Neurodegenerative Disorders.- In Vitro Brain Organoids and Computational Models to Study Cell Death in Brain Diseases.- Isolation of Mouse Embryonic Neural Stem Cells and Characterization of Neural Stem Markers by Flow Cytometry.- Assessment of Neuronal Cell Death in Caenorhabditis elegans.- Emerging Methods in Modeling Brain Development and Disease with Human Pluripotent Stem Cells.- Isolation of Adult Mouse Neural Stem Cells and Assessment of Self-Renewal by ELDA.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐