Fluorescence Spectroscopy and Microscopy in Biology

图书简介

This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.

Part I - Fluorescence Spectroscopy: Basics and Advanced Approaches.- Chapter 1 Choosing the right fluorescent probe.- Chapter 2 Fluorescence kinetics and time-resolved measurement.- Chapter 3 Quantitative Approach to Applications of Electronic Energy Transfer (EET).- Chapter 4 Single-molecule FRET: principles and analysis.- Chapter 5 Principles of fluorescence correlation and dual-color cross-correlation spectroscopy.- Part II - Fluorescence Microscopy: Basics and Advanced Approaches.- Chapter 6 Introduction to Fluorescence Microscopy.- Chapter 7 Wohland STED and RESOLFT fluorescent nanoscopy.- Chapter 8 Fluorescence Correlation Spectroscopy in Space and Time.- Part C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes.- Chapter 9 Determination of Biomolecular Oligomerization in the Live Cell Plasma Membrane via Single-molecule Brightness and Co-localization Analysis.- Chapter 10 Quantitative photoactivated localization microscopy of membrane receptor oligomers.- Chapter 11 Diffusion measurements at the nanoscale with STED-FCS.- Chapter 12 Single molecule microscopy methods to study mitochondrial processes.- Chapter 13 Transient state (TRAST) spectroscopy and imaging – exploiting the rich information source of fluorophore dark state transitions in biomolecular and cellular studies.- Chapter 14 The analysis of in-membrane nanoscopic aggregation of lipids and proteins by MC-FRET.- Part D - Applications of Fluorescence Spectroscopy to protein studies.- Chapter 15 Single-molecule fluorescence spectroscopy of intrinsically disordered proteins.- Chapter 16 Insights into the conformational dynamics of potassium channels using homo-FRET approaches.- Chapter 17 Intrinsic fluorescence kinetics in proteins.- Chapter 18 Dynamics and hydration of proteins viewed by fluorescence methods: investigations famor protein engineering and synthetic biology.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Fluorescence Spectroscopy and Microscopy in Biology（Springer Series on Fluorescence） 化学史

Fluorescence Spectroscopy and Microscopy in Biology（Springer Series on Fluorescence）
化学史