图书简介

Basic Methodology and Research Applications.- Quantitation of mRNA by the Polymerase Chain Reaction.- Sequencing of PCR Products ? Analysis of Factor IX Genes and of Recombination Events in Immunoglobulin Genes.- Alternative Methods for DNA Probing in Diagnosis: Ligase Chain Reaction (LCR).- Application of PCR to the Detection and Analysis of Point Mutations in the Human Factor VIII Gene.- The Use of PCR in the Diagnosis of Muscle Disease.- Taq DNA Polymerase-Synthesized Single-Stranded DNA Hybridization Probes and their Application in Northern Blotting and in situ Hybridization.- Computer Program for the Selection of PCR Primers.- Nervous System Cross Reactivity Sequences.- The Use of Short Adapters for Priming PCR of Unknown Chromosomal DNA Fragments.- Labeling of Specific DNA Sequences With Digoxigenin during Polymerase Chain Reaction.- Quantitative Analysis of Polymerase Chain Reaction (PCR) Products by Means of Fluorescence Detection.- Reverse Transcription PCR (RT-PCR) of Parathyroid Hormone (PTH) mRNA.- False Positive Reactions in PCR.- Error Production and Error Propagation During PCR.- Genetics and Evolution.- PCR in Linkage Analysis of Genetic Diseases.- Amplification, Isolation and Direct Nucleotide Determination of Entire Genes: Application to the Study of 16S rRNAs for Molecular Evolution in Bacteria, Identification of Cultural Isolates and Development of Probes.- Diagnosis of Cystic Fibrosis ? Detection of the Gene Deletion Delta F 508.- Application of PCR for Prenatal Diagnosis of Cystic Fibrosis (CF).- The Use of Non-Viable Cultured Amniotic Fluid Cells for Prenatal Diagnosis of Cystic Fibrosis by Polymerase Chain Reaction.- Determination of Glutathione S-Transferase u Genotype in Humans by PCR-Amplified Gene-Probes.- Rapid Apolipoprotein E Genotype Identification.- Detection and Characterization of Viral Infections.- Herpes Viruses.- Detection of Herpes Viruses by PCR ? Diagnostic Aspects.- Diagnosis of Herpes Simplex Virus Encephalitis (HSVE) by Polymerase Chain Reaction.- Rapid Detection of CMV-Specific DNA and mRNA by PCR in Immunocompromised Patients.- Specific and Sensitive Detection of Cytomegalovirus DNA in Clinical Specimens by PCR.- Detection of EBNA2-mRNA in Primary Central Nervous System Lymphomas in Patients with AIDS.- Detection of Epstein-Barr Virus Genomes in Lymphoproliferative Diseases.- Screening of Lymphatic Tissues for the Presence of Human Herpesvirus 6 DNA Using the Polymerase Chain Reaction (PCR).- Retroviruses.- HTLV Retrovirus in Neurological Disease.- Use of PCR to Investigate the Cellular Tropism of Human T Cell Leukemia Virus Type I.- Viral Genome Sequences in Cerebrospinal Fluid and Serum of HIV-1 Infected but Asymptomatic Patients.- Hepatitis Viruses.- PCR Detection of HBV Replication in Anti-HBeAg Chronic Carriers with Pre-S Antigenemia.- Detection of Virus DNA by PCR in Hepatitis Non-A, Non-B.- Hepatitis B Virus DNA ? Identification by Polymerase Chain Reaction (PCR) and Correlation with Serological HBV Markers.- Detection and Characterization of Bacterial Infections.- Rapid Diagnosis of Mycobacterium Tuberculosis Infection by PCR.- Detection of Chlamydia Trachomatis by PCR.- The Polymerase Chain Reaction (PCR) to Detect Gene Sequences of Borrelia Burgdorferi, the Etiologic Agent of Lyme Disease.- Rapid Diagnosis of Human Listeriosis Using the Polymerase Chain Reaction.- Oncogenes.- Quantitation of Mdr1 Transcript by PCR a Tool for Monitoring Drug Resistance in Cancer Chemotherapy.- Ras Oncogene Mutations in Renal Cell Cancer.- Primer Mismatch Analysis: a Rapid and Non-Isotopic Method for Analysing Ras Mutations in Human Cancer.- Expression of Adenovirus Type 12 13S Gene Product is Sufficient to Immortalize Primary Mouse Cells.- PCR-Based Rapid Detection of the Philadelphia-Chromosome in Chronic Myelogenous and Acute Lymphoblastic Leukemia.- List of Contributors.

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