Vertebrate Embryogenesis:Embryological, Cellular, and Genetic Methods

图书简介

This detailed volume explores animal embryogenesis in a way that aims to foster cross-model work and ideas by presenting methods that can be applied across laboratories and species boundaries. Improved protocols with updated advances in key traditional model systems are included, such as in amphibians, chicken, mouse, and zebrafish, as well as chapters on leopard gecko and the flexible-shelled slider turtle. Within these traditional model systems, new developments are presented, such as protocols for the analysis of cellular membranes and intracellular signals, light-controlled manipulation of function, and the analysis of transcriptomic and proteomic data in the context of the embryo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and fully updated, Vertebrate Embryogenesis: Embryological, Cellular, and Genetic Methods, Second Edition serves as an invaluable aid to scientists, educators, and the advanced general audience and will act as an inspiration to further our understanding and appreciation of animal development.

Chapter 10, ’Optogenetic Control of Subcellular Protein Location and Signalling in Vertebrate Embryos,’ is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Culture and Host Transfer of Xenopus Oocytes for Maternal mRNA Depletion and Genome Editing Experiments.- Immunofluorescence of Microtubule Assemblies in Amphibian Oocytes and Early Embryos.- Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos.- Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts.- Membrane Microdomains as Platform to Study Membrane-Associated Events during Oogenesis, Meiotic Maturation, and Fertilization in Xenopus laevis.- Embryological and Genetic Manipulation of Chick Development.- Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos.- Experimental Manipulation of Ploidy in Zebrafish Embryos and its Application in Genetic Screens.- RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq).- Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.- Ex Utero Culture and Imaging of Mouse Embryos.- Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.- Reptile Embryology and Regeneration.- Manipulation of Developmental Function in Turtles with Notes on Alligators.- Methods for Isolating the Balbiani Body/Germ Plasm from Xenopus laevis Oocytes.- Visualizing the Balbiani Body in Zebrafish Oocytes.- Isolation of Zebrafish Balbiani Bodies for Proteomic Analysis.- Generation of Xenopus Haploid, Triploid, and Hybrid Embryos.- Germ Cell Transplantation in Avian Species.- Production of Germ-Line Chimeras in Zebrafish.- A Method for Zebrafish Follicle Transplantation into Recipient Mothers for the Generation of Fertilizable Eggs and Viable Offspring.- Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT).- An Accessible Protocol for the Generation of CRISPR- Cas9 Knockouts Using INDELs in Zebrafish.- Modeling Embryonic Cleavage Patterns.- Laboratory Guidelines for Animal Care.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Vertebrate Embryogenesis:Embryological, Cellular, and Genetic Methods（Methods in Molecular Biology） 脊椎动物胚胎发生：胚胎学、细胞学与遗传学方法 第2版 发育生物学，古生物学

Vertebrate Embryogenesis:Embryological, Cellular, and Genetic Methods（Methods in Molecular Biology）

脊椎动物胚胎发生：胚胎学、细胞学与遗传学方法第2版
发育生物学，古生物学