图书简介
This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Oncolytic Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Introduction to Oncolytic Virotherapy.- Methods for Modification of Therapeutic Viruses.- Tumor Targeting of Oncolytic Adenoviruses using Bispecific Adapter Proteins.- Development of Entry-targeted Oncolytic Measles Viruses.- Insert Stability and In Vivo Testing of MicroRNA-Detargeted Oncolytic Picornaviruses.- Ex vivo Virotherapy with Myxoma Virus to Treat Cancer.- Immunomodulation in Oncolytic Measles Virotherapy.- A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death.- Design and Production of Newcastle Disease Virus for Intratumoral Immunomodulation.- Analysis of Immunological Treatment Effects of Virotherapy in Tumor Tissue.- Immunohistochemistry for Tumor-infiltrating Immune Cells after Oncolytic Virotherapy.- Detection of Tumor Antigen-specific T-cell Responses after Oncolytic Vaccination.- Evaluation of Oncolytic Virus Induced Therapeutic Tumor Vaccination Effects in Murine Tumor Models.- Delivery of Oncolytic Reovirus by Cell Carriers.- In Vivo Bioimaging for Monitoring Intratumoral Virus Activity.- Oncolytic Immunotherapy for Bladder Cancer Using Coxsackie A21 virus: Using a Bladder Tumour Precision-cut Slice Model System to Assess Viral Efficacy.- Use of Liquid Patient Ascites Fluids as a Pre-clinical Model for Oncolytic Virus Activity.- Generating Primary Models of Human Cancer to Aid in the Development of Clinically-relevant Oncolytic Viruses.- Considerations for Clinical Translation of MG1 Maraba Virus.- Fluorescence in Situ Hybridization (FISH) Detection of Viral Nucleic Acids in Oncolytic H-1 Parvovirus-treated Human Brain Tumors.- Mathematical Modeling of Oncolytic Virotherapy.
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