Imaging Mass Spectrometry:Methods and Protocols

图书简介

This volume addresses the various techniques and novel applications of mass spectrometry imaging (MSI) and its role as a discovery tool in the field of proteomics, lipidomics, and metabolomics. The chapters in this book demonstrate how MSI can be applied to many areas of research such as clinical pathology, translational medicine, toxicology, biomarkers and response studies, and potential incorporation of MSI into forensic workflows. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Innovative and comprehensive, Imaging Mass Spectrometry: Methods and Protocols is a valuable resource for research scientists and clinicians who are interested in further studies of MSI technologies.

?A Future Amalgamation between the Scientist and the Clinician??.- Fresh Frozen Versus Formalin Fixed Paraffin Embedded for Mass Spectrometry Imaging.- Imaging MS of Rodent Ocular Tissues and the Optic Nerve.- MALDI-MSI of Lipids in Human Skin.- MALDI-MSI Analysis of Cytological Smears: The Study of Thyroid Cancer.- Droplet-Based Liquid Extraction for Spatially-Resolved Microproteomics Analysis of Tissue Sections.- DESI Mass Spectrometry Imaging (MSI).- Peptide Imaging: Maximizing Peptide Yield, Optimization of the ?Peptide Mass Fingerprint?.- MALDI-MS Imaging in the Study of Glomerulonephritis.- Hierarchical Cluster Analysis to Aid Diagnostic Image Data Visualization of MS and Other Medical Imaging Modalities.- Laser Ablation Inductively Coupled Plasma Mass Spectrometry Imaging of Plant Metabolites.- Mass Spectrometry Imaging of Drugs of Abuse in Hair.- MALDI Mass Spectrometry Profiling and Imaging Applied to the Analysis of Latent Fingermarks.- ToF-SIMS Parallel Imaging MS/MS of Lipid Species in Thin Tissue Sections.- Rodent Whole-Body Sectioning and MALDI Mass Spectrometry Imaging.- The Future in Disease Models for Mass Spectrometry Imaging, Ethical Issues and the Way Forward.

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Imaging Mass Spectrometry:Methods and Protocols（Methods in Molecular Biology） 放射医学

Imaging Mass Spectrometry:Methods and Protocols（Methods in Molecular Biology）
放射医学