图书简介
Listeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Protocols addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation and finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Vital and authoritative, Listeria monocytogenes: Methods and Protocols aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research.
Sampling the Production/Processing Environment for Listeria.- Traditional Methods for Isolation of Listeria monocytogenes.- Confirmation of Isolates of Listeria by Conventional and Real-Time PCR.- Serotype Assignment by Sero-Agglutination, ELISA and PCR.- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes.- Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes.- Ribotyping and Automated Ribotyping of Listeria monocytogenes.- Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes.- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray.- Analysis of Listeria monocytogenes Subproteomes.- The Listeria Cell Wall and Associated Carbohydrate Polymers.- Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria.- Virulence Characterization of Listeria monocytogenes.- Internalization Assays for Listeria monocytogenes.- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes.- Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the ?SOEing? Method.- Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes.- Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR.- Genome Sequencing of Listeria monocytogenes.- Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels.- Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers.- Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique.- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐