图书简介
In recent years, large-scale advances in technology have led to greater understanding of the world on a much tinier scale: the biomolecular level. In Micro and Nano Technologies in Bioanalysis: Methods and Protocols, expert researchers from across the globe explore the technology which makes this analysis possible, investigating the worlds of microfluidics and nanotechnologies, and examining physical science techniques for the separation, detection, manipulation, and analysis of biomolecules. This volume contains innovative protocols on the application of microfluidics and the utilization of physical science-related technologies that will prove to be invaluable in the field of molecular biology. Chapters contain cutting edge applications of emerging nanotechnologies, including quantum dots and molecular fluorescence for the imaging and tracking of biomolecules. Composed in the highly successful Methods in Molecular Biology? series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and groundbreaking, Micro and Nano Technologies in Bioanalysis: Methods and Protocols is a necessary tool for cellular biologists, biochemists, microbiologists, geneticists and medical researchers alike.
Applications of microfluidics and nanopores in separation, detection, manipulation, and analysis of biomolecules.- HPLC-Chip/MS Technology in Proteomic Profiling.- Nanofluidic Channel Fabrication and Manipulation of DNA Molecules.- A Single-Molecule Barcoding System using Nanoslits for DNA Analysis.- Microfluidic Devices with Photodefinable Pseudo-valves for Protein Separation.- Microfluidic Chips Designed for Measuring Biomolecules Through a Microbead-Based Quantum Dot Fluorescence Assay.- DNA Focusing Using Microfabricated Electrode Arrays.- Solid-State Nanopore for Detecting Individual Biopolymers.- Inserting and Manipulating DNA in a Nanopore with Optical Tweezers.- Forming an ?-Hemolysin Nanopore for Single-Molecule Analysis.- Nanopore Force Spectroscopy on DNA Duplexes.- Technologies of physical science and chemistry in detection and analysis of biomolecules.- Quantitative Chemical Analysis of Single Cells.- Trapping and Detection of Single Molecules in Water.- ZnO Nanorods as an Intracellular Sensor for pH Measurements.- Analysis of Biomolecules Using Surface Plasmons.- Use of Residual Dipolar Couplings in Structural Analysis of Protein-Ligand Complexes by Solution NMR Spectroscopy.- Raman-Assisted X-Ray Crystallography for the Analysis of Biomolecules.- Methods and Software for Diffuse X-Ray Scattering from Protein Crystals.- Deuterium Labeling for Neutron Structure-Function-Dynamics Analysis.- Small-Angle Neutron Scattering for Molecular Biology: Basics and Instrumentation.- Small-Angle Scattering and Neutron Contrast Variation for Studying Bio-Molecular Complexes.- Protein Sequencing with Tandem Mass Spectrometry.- Metabolic Analysis.- Applications of quantum dots and molecular fluorescence in detection, tracking and imaging of biomolecules.- Multicolor Detection of Combed DNA Molecules Using Quantum Dots.- Quantum Dot Molecular Beacons for DNA Detection.- Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Interactions.- In Vivo Imaging of Quantum Dots.- Semiconductor Fluorescent Quantum Dots: Efficient Biolabels in Cancer Diagnostics.- The Monitoring and Affinity Purification of Proteins Using Dual Tags with Tetracysteine Motifs.- Use of Genomic DNA as Reference in DNA Microarrays.- Single-Molecule Imaging of Fluorescent Proteins Expressed in Living Cells.- MicroPET, MicroSPECT, and NIR Fluorescence Imaging of Biomolecules In Vivo.- Ultrahigh Resolution Imaging of Biomolecules by Fluorescence Photoactivation Localization Microscopy.- Nanotechnologies for biomolecular delivery, gene therapy and expression control.- Real-Time Imaging of Gene Delivery and Expression with DNA Nanoparticle Technologies.- Nanoparticle-Mediated Gene Delivery.- Magnetic Nanoparticles for Local Drug Delivery Using Magnetic Implants.- Functionalized Magnetic Nanoparticles as an In Vivo Delivery System.- Formulation/Preparation of Functionalized Nanoparticles for In Vivo Targeted Drug Delivery.- Detection of mRNA in Single Living Cells Using AFM Nanoprobes.- Reverse Transfection Using Gold Nanoparticles.- Custom-Designed Molecular Scissors for Site-Specific Manipulation of the Plant and Mammalian Genomes.- Determining DNA Sequence Specificity of Natural and Artificial Transcription Factors by Cognate Site Identifier Analysis.
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