图书简介
This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide a sound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.
Introduction to Purinergic Signaling.- Knock-out and Knock-in Mouse Models to Study Purinergic Signaling.- Agonists and Antagonists for Purinergic Receptors.- Homology Modelling of P2X Receptors.- Using RNA Interference for Purinoceptor Knock Down in vivo.- Developmental Expression of Ectonucleotidase and Purinergic Receptors Detection by Whole Mount in situ hybridization in Xenopus Embryos.- Histochemical Approach for Simultaneous Detection of Ecto-nucleotidase and Alkaline Phosphatase Activities in Tissues.- Flow Cytometry of Membrane Purinoreceptors.- Studying Purinoceptor Cell-surface Expression by Protein Biotinylation.- Multimeric Ionotropic Purinoceptor Detection byProtein Cross-linking.- Multimeric Purinoceptor Detection by BioluminiscenceResonance Energy Transfer.- Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy.- Detection of Extracellular ATP in the Tumor Microenvironment, using the pmeLUC Biosensor.- Using Amperometric, Enzyme-Based Biosensors for Performing Longitudinal Measurements of Extracellular adenosine 5-triphosphate in the Mouse.- Fluorescent Labeling and Quantification of Vesicular ATP Release using Live Cell Imaging.- Using FRET-based Fluorescent Sensors to Monitor Cytosolic and Membrane-proximal Extracellular ATP levels .- ATP Measurement in Cerebrospinal Fluid Using a Microplate Reader.- P2X Electrophysiology and Surface Trafficking in Xenopus oocytes.- Heterologous Expression and Patch-clamp Recording of P2X Receptors in HEK293 Cells.- Recording P2X Receptors Using Whole Cell Patch Clamp from Native Monocytes and Macrophages.- Automated Planar Patch-clamp Recording of P2X Receptors.- Controlling Engineered P2X Receptors with Light.- Intracellular Calcium Recording after Purinoceptor Activation Using a Video-microscopy Equipment.- Assays to Measure Purinoceptor Pore Dilation.- Detection of Inflammasome Activation by P2X7 Purinoceptor Activation by Determining ASC Oligomerization.- Measuring Leukocyte Migration to Nucleotides.- Assessment of Cell Adhesion after Purinoceptor Activation.
Trade Policy 买家须知
- 关于产品:
- ● 正版保障:本网站隶属于中国国际图书贸易集团公司,确保所有图书都是100%正版。
- ● 环保纸张:进口图书大多使用的都是环保轻型张,颜色偏黄,重量比较轻。
- ● 毛边版:即书翻页的地方,故意做成了参差不齐的样子,一般为精装版,更具收藏价值。
关于退换货:
- 由于预订产品的特殊性,采购订单正式发订后,买方不得无故取消全部或部分产品的订购。
- 由于进口图书的特殊性,发生以下情况的,请直接拒收货物,由快递返回:
- ● 外包装破损/发错货/少发货/图书外观破损/图书配件不全(例如:光盘等)
并请在工作日通过电话400-008-1110联系我们。
- 签收后,如发生以下情况,请在签收后的5个工作日内联系客服办理退换货:
- ● 缺页/错页/错印/脱线
关于发货时间:
- 一般情况下:
- ●【现货】 下单后48小时内由北京(库房)发出快递。
- ●【预订】【预售】下单后国外发货,到货时间预计5-8周左右,店铺默认中通快递,如需顺丰快递邮费到付。
- ● 需要开具发票的客户,发货时间可能在上述基础上再延后1-2个工作日(紧急发票需求,请联系010-68433105/3213);
- ● 如遇其他特殊原因,对发货时间有影响的,我们会第一时间在网站公告,敬请留意。
关于到货时间:
- 由于进口图书入境入库后,都是委托第三方快递发货,所以我们只能保证在规定时间内发出,但无法为您保证确切的到货时间。
- ● 主要城市一般2-4天
- ● 偏远地区一般4-7天
关于接听咨询电话的时间:
- 010-68433105/3213正常接听咨询电话的时间为:周一至周五上午8:30~下午5:00,周六、日及法定节假日休息,将无法接听来电,敬请谅解。
- 其它时间您也可以通过邮件联系我们:customer@readgo.cn,工作日会优先处理。
关于快递:
- ● 已付款订单:主要由中通、宅急送负责派送,订单进度查询请拨打010-68433105/3213。
本书暂无推荐
本书暂无推荐