图书简介

Purification of Mammalian Tubulins and Tubulin-Associated Proteins using a P2A-based Expression System.-Expression and Purification of Microtubule Associated Proteins from HEK293T Cells for In Vitro Reconstitution.-In vitro Reconstitution Assays of Microtubule Amplification and Lattice Repair by the Microtubule Severing Enzymes.-In vitro Microtubule Dynamics Assays using Darkfield Microscopy.-In vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Laments in Emulsion Droplets.-Studying Tau-microtubule Interaction using Single Molecule TIRF Microscopy.-In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis.-Methods for Studying Membrane-associated Bacterial Cytoskeleton Proteins in vivo by TIRF Microscopy.-Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-like Assemblies.-Studying Centriole Duplication and Elongation in Human Cells.-Assaying Microtubule Nucleation.-Detection of Microtubule Nucleation Hotspots at the Golgi.-Ultrastructural Analysis of Microtubule Ends.-Optogenetic Control of Microtubule Dynamics.-Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation of Fluorescent Tubulin.-Functional Dissection of Mitosis using Immortalized Fibroblasts from the Indian Muntjac, A Placental Mammal with only Three Chromosomes.-Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes.-Using the Four-cell C. elegans Embryo to Study Contractile Ring Dynamics during Cytokinesis.-Knocking out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifications.-Measuring the Role of Tubulin Posttranslational Modifications on Axonal Transport.-Methods to Measure Perinuclear Actin Dynamics during Nuclear Movement in Migrating Cells.

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