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Fertilization When sperm meets egg-Fifty years of surprises David Epel Early and later studies on action potential and fertilization potential of echinoderm oocytes and Ca2+ response of mammalian oocytes Shunichi Miyazaki My research career on (mainly) sea urchins Victor D. Vacquier Cytokenesis and Embryology Echinoderm eggs as a model for discoveries in cell biology David R. Burgess Unlocking mechanisms of development through advances in tools David McClay From hemoglobin to urchin spicules Fred Wilt Genomics and Morphogenesis The causes of things Robert D. Burke A personal history of the echinoderm genome sequencing R. Andrew Cameron Section 1 - Methods for genome and transcriptome analysis Genomic resources for the study of echinoderm development and evolution Gregory A. Cary, R. Andrew Cameron and Veronica F. Hinman Methods for the experimental and computational analysis of gene regulatory networks in sea urchins Isabelle S. Peter Using ATAC-seq and RNA-seq to increase resolution in GRN connectivity Elijah K. Lowe, Claudia Cuomo, Danila Voronov and Maria I. Arnone Identifying gene expression from single cells to single genes Nathalie Oulhen, Stephany Foster, Greg Wray and Gary Wessel Multiplex cis-regulatory analysis Jongmin Nam Whole mount in situ hybridization techniques for analysis of the spatial distribution of mRNAs in sea urchin embryos and early larvae Eric M. Erkenbrack, Jenifer C. Croce, Esther Miranda, Sujan Gautam, Marina Martinez-Bartolome, Shunsuke Yaguchi and Ryan C. Range Techniques for analyzing gene expression using BAC-based reporter constructs Katherine M. Buckley and Charles A. Ettensohn Genome-wide analysis of chromatin accessibility using ATAC-seq Tanvi Shashikant and Charles A. Ettensohn Section 2 - Genome-editing and proteomics Expression of exogenous mRNAs to study gene function in echinoderm embryos Maria Dolores Molina, Christian Gache and Thierry Lepagef Trapping, tagging and tracking: Tools for the study of proteins during early development of the sea urchin Michelle M. Roux-Osovitz, Kathy R. Foltz, Nathalie Oulhen and Gary Wessel CRISPR/Cas9-mediated genome editing in sea urchins Che-Yi Lin, Nathalie Oulhen, Gary Wessel and Yi-Hsien Su Analysis of microRNA functions Carolyn Remsburg, Kalin Konrad, Nina Faye Sampilo and Jia L. Song In vivo analysis of protein translation activity in sea urchin eggs and embryos Heloise Chasse, Sandrine Boulben, Virginie Glippa, Florian Pontheaux, Patrick Cormier and Julia Morales Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos Catherine S. Schrankel, Tufan Goekirmak, Chang-Wook Lee, Geoffrey Chang and Amro Hamdoun Section 3 - Imaging of echinoderm embryos Live-cell fluorescence imaging of echinoderm embryos Silvia P. Sepulveda-Ramirez, Leslie Toledo-Jacobo, Chelsea Garno, Debadrita Pal, Clara Ross, Andrea Ellis and Charles B. Shuster 3D + time imaging of normal and twin sea urchin embryos for the reconstruction of their cell lineage Antonio Ortiz, Elena Kardash and Nadine Peyrieras High resolution imaging of the cortex isolated from sea urchin eggs and embryos J.H. Henson, Bakary Samasa and E.C. Burg Spatially mapping gene expression in sea urchin primary mesenchyme cells Daniel T. Zuch and Cynthia A. Bradham Section 4 - Methods for measurement of intracellular signals in eggs, sperm and embryos Probing Ca2+ release mechanisms using sea urchin egg homogenates Yu Yuan, Gihan S. Gunaratne, Jonathan S. Marchant and Sandip Patel Measuring voltage and ion concentrations in live embryos Nahomie Rodriguez-Sastre, Christopher F. Thomas and Cynthia A. Bradham Analysis of sperm chemotaxis Hector Vicente Ramirez-Gomez, Idan Tuval, Adan Guerrero and Alberto Darszon Kinetic and photonic techniques to study chemotactic signaling in sea urchin sperm Hussein Hamzeh, Luis Alvarez, Timo Strunker, Michelina Kierzek, Christoph Brenker, Parker E. Deal, Evan W. Miller, Reinhard Seifert and U. Benjamin Kaupp Analysis of neural activity with fluorescent protein biosensors Robert D. Burke and Shunsuke Yaguchi

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