图书简介

The Simplest Protocol for Rapid and Long-Term Culture of Primary Epidermal Keratinocytes from Human and Mouse.- Dermal-Epidermal Separation by Heat.- Dermal-Epidermal Separation by Enzyme.- Dermal-Epidermal Separation by Chemical.- Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures.- Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential.- Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis.- Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte.- Digital Quantification of Epidermal Protein Expression in Paraffin-Embedded Tissue Using Immunohistochemistry.- Keratinocyte Differentiation by Flow Cytometry.- In Silico and In Vitro Considerations of Keratinocyte Nuclear Receptor Protein Structural Order for Improving Experimental Analysis.- Genetic Modification of Human Primary Keratinoctes by Lentiviral Vectors.- Generation of Knockout Human Primary Keratinocytes by CRISPR/Cas9.- In Vitro Wound Healing Assays to Investigate Epidermal Migration.- Iterative Three-Dimensional Epidermis Bioengineering and Xenografting to Assess Long-Term Regenerative Potential in Human Keratinocyte Precursor Cells.- Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse.- Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures.- Information and Statistical Analysis Pipeline for High-Throughput RNA Sequencing Data.- Cell-Extracellular Matrix Adhesion Assay.- Methods for Analysis of Keratinocyte Migration.- Scratch Wound Healing Assay.- Photoprotective Activity Assay toward Ultraviolet B in Human Keratinocytes.- Photodynamic Therapy Assay.- A Method to Prepare Claudin-Modulating Recombinant Proteins.- Human Fetal Skin Fibroblast Isolation and Expansion for Clinical Application.- Ciliated Epithelial Cell Differentiation at Air-Liquid Interface Using Commercially Available Culture Media.

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